Original Research Articles
Vol. 31 No. 2 (2024)
Photo-disinfection of orthodontic brackets contaminated with Lactobacillus acidophilus with blue laser
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 18 August 2024
Accepted: 12 October 2024
Accepted: 12 October 2024
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Decontamination of teeth with Chlorhexidine (CHX) in the treatment of dental disease is associated with some concerns. The objective of the current study was to ascertain whether the Blue Diode Laser (BDL), as a new approach in combination with riboflavin and curcumin as photosensitizers, would have any impact on the number of Lactobacillus acidophilus around orthodontic brackets. A total of 36 orthodontic brackets were contaminated with L. acidophilus and categorized into six different groups, including the negative control, riboflavin alone or riboflavin + BDL with a radiant power of 500 mW, and curcumin alone or curcumin + BDL with a radiant power of 500 mW, and 0.2% CHX as positive control. Orthodontic brackets were irradiated with a BDL (wavelength of 450 nm) and radiant exposure of 30 J/cm2 for 30 s. Colony-forming units per milliliter (CFUs/ml) were determined. One-way Analysis Of Variance (ANOVA) followed by Tukey’s post-hoc tests were performed to compare CFU/ml between groups. All groups were better at eliminating L. acidophilus around orthodontic brackets than the negative control group, but this was not significant for riboflavin alone. The curcumin groups were more effective than the riboflavin groups at reducing CFU/ml of L. acidophilus. In addition, CHX was able to completely eliminate the colonies of L. acidophilus (p <0.0001). This study showed that curcumin and riboflavin plus BDL significantly reduced the amounts of L. acidophilus around the orthodontic brackets.
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Ethics Approval
The study protocol was reviewed and approved by the Ethics Committee of the Tehran University of Medical Sciences (IR. TUMS. DENTISTRY.REC. 1400. 187). All teeth were extracted because of orthodontic reasons and the owners of the teeth agreed to the donation.CRediT authorship contribution
All authors contributed equally to the work. All authors read and approved the final version of the manuscript and agreed to be accountable for all aspects of the work.
Data Availability Statement
All data generated or analyzed during this study are included in this published article.
How to Cite
Photo-disinfection of orthodontic brackets contaminated with Lactobacillus acidophilus with blue laser. (2024). Laser Therapy, 31(2). https://doi.org/10.4081/ltj.2024.404
Copyright (c) 2024 the Author(s)
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