https://doi.org/10.4081/ltj.2022.306

Chitosan adhesives with sub-micron structures for photochemical tissue bonding

Vol. 29 No. 2 (2022)
Published: 27 December 2022
Nanostructures, nanofabrication, tissue repair, tissue engineering
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Authors

Samuel J. Frost
The College, Western Sydney University, NSW, Australia.
Jessica Houang
School of Science, Western Sydney University, Penrith, NSW, Australia.
James M. Hook
School of Chemistry, University of New South Wales, Sydney, NSW, Australia.
Antonio Lauto
A.Lauto@westernsydney.edu.au
School of Science, Western Sydney University, Penrith, NSW, Australia.

We describe a method for fabricating biocompatible chitosan-based adhesives with sub-micron structures to enhance tissue bonding. This procedure avoids coating and chemical modification of structures and requires a simple drop-casting step for the adhesive film formation. Chitosan thin films (27±3 μm) were fabricated with sub-micron pillars (rectangular cuboid with height ∼150 nm, square dimension ∼1 μm and pitch ∼2 μm) or holes (diameter ~500 nm or ~1 μm, depth ~100 or 400 nm, pitch of 1 or 2 μm). Polydimethylsiloxane moulds were used as negative templates for the adhesive solution that was cast and then allowed to dry to form thin films. These were applied on bisected rectangular strips of small sheep intestine and photochemically bonded by a green laser (λ= 532 nm, irradiance ∼110 J/cm2). The tissue repair was subsequently measured using a computer-interfaced tensiometer. The mould sub-micron structures were reproduced in the chitosan adhesive with high fidelity. The adhesive with pillars achieved the highest bonding strength (17.1±1.2 kPa) when compared to the adhesive with holes (13.0±1.3 kPa, p<0.0001, one-way ANOVA, n=15). The production of chitosan films with patterned pillars or holes in the sub-micron range was demonstrated, using a polydimethylsiloxane mould and a single drop-casting step. This technique is potentially scalable to produce adhesives of larger surface areas.

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Citations

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Scopus
Google Scholar
Europe PMC
Barton M, Morley JW, Stoodley MA, et al. Laser-activated adhesive films for sutureless median nerve anastomosis: laser-activated adhesive films. J Biophotonics 2013;6:938–49. DOI: https://doi.org/10.1002/jbio.201300054
Barton MJ, Morley JW, Stoodley MA, et al. Long term recovery of median nerve repair using laser-activated chitosan adhesive films: sutureless median nerve repair. J Biophotonics 2015;8:196–207. DOI: https://doi.org/10.1002/jbio.201300129
Sliow A, Ma Z, Gargiulo G, et al. Stimulation and Repair of Peripheral Nerves Using Bioadhesive Graft-Antenna. Adv Sci 2019;6:1801212. DOI: https://doi.org/10.1002/advs.201801212
Shapiro AJ, Dinsmore RC, North JHJ. Tensile strength of wound closure with cyanoacrylate glue. Am Surg 2001;67:1113–5. DOI: https://doi.org/10.1177/000313480106701118
Komatsu F, Mori R, Uchio Y. Optimum surgical suture material and methods to obtain high tensile strength at knots: problems of conventional knots and the reinforcement effect of adhesive agent. J Orthop Sci Off J Japanese Orthop Assoc 2006;11:70–4. DOI: https://doi.org/10.1007/s00776-005-0973-x
Varenberg M, Gorb S. A beetle-inspired solution for underwater adhesion. J R Soc Interface 2008;5:383–5. DOI: https://doi.org/10.1098/rsif.2007.1171
Gorb SN. Biological attachment devices: exploring nature’s diversity for biomimetics. Philos Trans R Soc A Math Phys Eng Sci 2008;366:1557–74. DOI: https://doi.org/10.1098/rsta.2007.2172
Autumn K, Liang YA, Hsieh ST, et al. Adhesive force of a single gecko foot-hair. Nature 2000;405:681–5. DOI: https://doi.org/10.1038/35015073
Rong Z, Zhou Y, Chen B, et al. Bio-inspired hierarchical polymer fiber-carbon nanotube adhesives. Adv Mater 2014;26:1456–61. DOI: https://doi.org/10.1002/adma.201304601
Hu H, Wang D, Tian H, et al. Bioinspired hierarchical structures for contact-sensible adhesives. Adv Funct Mater 2022;32:2109076. DOI: https://doi.org/10.1002/adfm.202109076
Shi W, Cheng X, Cheng K. Gecko-inspired adhesives with asymmetrically tilting-oriented micropillars. Langmuir 2022;38:8890–8. DOI: https://doi.org/10.1021/acs.langmuir.2c01002
Bergström L. Hamaker constants of inorganic materials. Adv Colloid Interface Sci 1997;70:125–69. DOI: https://doi.org/10.1016/S0001-8686(97)00003-1
Lee H, Lee BP, Messersmith PB. A reversible wet/dry adhesive inspired by mussels and geckos. Nature 2007;448:338–41. DOI: https://doi.org/10.1038/nature05968
Mahdavi A, Ferreira L, Sundback C, et al. A biodegradable and biocompatible gecko-inspired tissue adhesive. Proc Natl Acad Sci 2008;105:2307–12. DOI: https://doi.org/10.1073/pnas.0712117105
Pereira MJN, Sundback CA, Lang N, et al. Combined surface micropatterning and reactive chemistry maximizes tissue adhesion with minimal inflammation. Adv Healthc Mater 2014;3:565–71. DOI: https://doi.org/10.1002/adhm.201300264
Moreira Lana G, Sorg K, Wenzel GI, et al. Self-adhesive silicone microstructures for the treatment of tympanic membrane perforations. Adv NanoBiomed Res 2021;1:2100057. DOI: https://doi.org/10.1002/anbr.202100057
Frost SJ, Mawad D, Higgins MJ, et al. Gecko-inspired chitosan adhesive for tissue repair. NPG Asia Mater 2016;8:e280. DOI: https://doi.org/10.1038/am.2016.73
Lauto A, Stoodley M, Barton M, et al. Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair. J Vis Exp 2012:e4158. DOI: https://doi.org/10.3791/4158
Lauto A, Mawad D, Barton M, et al. Photochemical tissue bonding with chitosan adhesive films. Biomed Eng Online 2010;9:1–11. DOI: https://doi.org/10.1186/1475-925X-9-47
Barton MJ, Morley JW, Mahns DA, et al. Tissue repair strength using chitosan adhesives with different physical-chemical characteristics. J Biophotonics 2014;8:1–8. DOI: https://doi.org/10.1002/jbio.201300148
Glass P, Chung H, Washburn NR, Sitti M. Enhanced wet adhesion and shear of elastomeric micro-fiber arrays with mushroom tip geometry and a photopolymerized p(DMA-co-MEA) tip coating. Langmuir 2010;26:17357–62. DOI: https://doi.org/10.1021/la1029245
Glass P, Chung H, Washburn NR, Sitti M. Enhanced reversible adhesion of dopamine methacrylamide-coated elastomer microfibrillar structures under wet conditions. Langmuir 2009;25:6607–12. DOI: https://doi.org/10.1021/la9009114
Fernandez JG, Mills CA, Samitier J. Complex microstructured 3D surfaces using chitosan biopolymer. Small 2009;5:614–20. DOI: https://doi.org/10.1002/smll.200800907

How to Cite

Frost, S. J., Houang, J., Hook, J. M., & Lauto, A. (2022). Chitosan adhesives with sub-micron structures for photochemical tissue bonding. Laser Therapy, 29(2). https://doi.org/10.4081/ltj.2022.306
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