Original Research Articles
Vol. 32 No. 1 (2025)
In vivo pilot study of the effects of a subdermal 1470 nm diode laser on human skin
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Published: 30 June 2025
796
Views
475
Downloads
Authors
This in vivo pilot study investigates the histological regenerative effects of a subdermal 1470 nm laser (EndoliftX®, Eufoton®, Trieste, Italy) on human abdominal skin, aiming to assess its role in dermal remodeling. Laser energy was delivered at 5, 20, and 40 J/cm2 to defined areas of abdominal skin in patients undergoing abdominoplasty. Biopsies were obtained at 3 and 6 months post-treatment and assessed for changes in dermal thickness, collagen, and elastin content compared to the control untreated region. Histological staining and image analysis revealed that the 5 and 20 J/cm2 treatments significantly increased dermal thickness and collagen deposition, particularly with the 20 J/cm2 dose, which induced dense, organized collagen bundles indicative of neocollagenesis. Sirius red staining showed increased type III collagen, and Verhoeff-Van Gieson (VVG) staining indicated enhanced and more aligned elastin fibers at moderate energy levels. Conversely, the 40 J/cm2 treatment dose showed signs of collagen fragmentation and reduced elastin coherence, suggesting potential thermal damage. These findings confirm the efficacy of EndoliftX in promoting skin tightening and remodeling at optimal energy settings while highlighting a non-linear dose-response relationship. The results support further development of personalized protocols for minimally invasive procedures in regenerative medicine and aesthetic dermatology.
Downloads
Download data is not yet available.
Citations
1. Modena DAO, Miranda ACG, Grecco C, et al. Efficacy, safety, and guidelines of application of the fractional ablative laser erbium YAG 2940 nm and non-ablative laser erbium glass in rejuvenation, skin spots, and acne in different skin phototypes: a systematic review. Lasers Med Sci 2020;35:1877-88. DOI: https://doi.org/10.1007/s10103-020-03046-7
2. Shanina NA, Patrushev AV, Zorman A. Histological and immunohistochemical changes in facial skin treated with combined ablative and non-ablative laser therapy. J Cosmet Dermatol 2021;20:3509-16. DOI: https://doi.org/10.1111/jocd.14023
3. Leszczynski R, da Silva CA, Pinto ACPN, et al. Laser therapy for treating hypertrophic and keloid scars. Cochrane Database Syst Rev 2022 26;9:CD011642. DOI: https://doi.org/10.1002/14651858.CD011642.pub2
4. Lai D, Zhou S, Cheng S, et al. Laser therapy in the treatment of melasma: a systematic review and meta-analysis. Lasers Med Sci 2022;37:2099-110. DOI: https://doi.org/10.1007/s10103-022-03514-2
5. Haykal D, Cartier H, Goldberg D, Gold M. Advancements in laser technologies for skin rejuvenation: A comprehensive review of efficacy and safety. J Cosmet Dermatol 2024;23:3078-89. DOI: https://doi.org/10.1111/jocd.16514
6. Zelickson BD, Dressel TD. Discussion of laser-assisted liposuction. Lasers Surg Med 2009;41:709-13. DOI: https://doi.org/10.1002/lsm.20842
7. Sasaki GH, Tevez A. Laser-assisted liposuction for facial and body contouring and tissue tightening: a 2-year experience with 75 consecutive patients. Semin Cutan Med Surg 2009;28:226-35. DOI: https://doi.org/10.1016/j.sder.2009.10.003
8. Pereira-Netto D, Montano-Pedroso JC, Aidar ALS, et al. Laser-Assisted Liposuction (LAL) versus traditional liposuction: systematic review. Aesthetic Plast Surg 2018;42:376-83. DOI: https://doi.org/10.1007/s00266-018-1085-2
9. Goldman A, Gotkin RH. Laser-assisted liposuction. Clin Plast Surg 2009;36:241-53. DOI: https://doi.org/10.1016/j.cps.2008.11.005
10. Weiss RA. Laser lipolysis and laser-assisted liposuction. In Fat Removal: invasive and non-invasive body contouring; Wiley-Blackwell: Hoboken, NJ, USA, 2015; pp. 120-36. DOI: https://doi.org/10.1002/9781118661932.ch8
11. Kim KH, Geronemus RG. Laser lipolysis using a novel 1,064 nm Nd:YAG laser. Dermatol Surg 2006;32:241-8. DOI: https://doi.org/10.1111/j.1524-4725.2006.32041.x
12. Heller L, Menashe S, Plonski L, et al. 1470-nm Radial fiber-assisted liposuction for body contouring and facial fat grafting. J Cosmet Dermatol 2022;21:1514-22. DOI: https://doi.org/10.1111/jocd.14767
13. Seitz M, Ruszat R, Bayer T et al. Ex vivo and in vivo investigations of the novel 1,470 nm diode laser for potential treatment of benign prostatic enlargement. Lasers Med Sci 2009;24:419-24. DOI: https://doi.org/10.1007/s10103-008-0591-x
14. Zerweck C, von Hodenberg E, Knittel M, et al. Endovenous laser ablation of varicose perforating veins with the 1470-nm diode laser using the radial fibre slim. Phlebology 2014;29:30-6. DOI: https://doi.org/10.1258/phleb.2012.012072
15. Dell’Avanzato R. Endolift®: the “lunch-time” laser lifting for the lower eyelids. Laser Therapy 2022;29:157-9. DOI: https://doi.org/10.4081/ltj.2022.307
16. Longo L, Dell’Avanzato R, Longo D. ENDOLIFT® and multi-wavelength laser photobiomodulation: a randomized controlled trial study on 96 subjects, treating skin laxity of the lower third of the face. Laser Therapy 2022;29:115-20. DOI: https://doi.org/10.4081/ltj.2022.298
17. Scrimali L, Lomeo G, Dell’Avanzato R, Crippa A. Endolaser soft-lift: a new approach on body contouring. Perspective and suggestions. Eur J Aesth Med Dermatol 2013;3:86-90.
18. Nilforoushzadeh MA, Heidari A, Ghane Y, et al. The Endo-lift Laser (Intralesional 1470 nm Diode Laser) for Dermatological Aesthetic Conditions: A Systematic Review. Aesth Plast Surg 2024;48:5097-114. DOI: https://doi.org/10.1007/s00266-024-04082-2
19. Modena DAO, de Melo Yamamoto AP, da Silva TBF. Endolift® is a non-surgical treatment for skin tissue conditions. Is there evidence for its application? Lasers Med Sci 2025;40:22. DOI: https://doi.org/10.1007/s10103-025-04288-z
20. Lotfi E, Dell’Avanzato R, Ahramiyanpour N, et al. Evaluation of eyebrow position and upper eyelid laxity after endolift laser treatment. Skin Res Technol 2023;29:e13498. DOI: https://doi.org/10.1111/srt.13498
21. Markabayeva A, Kaliterna D, Karimov S, et al. Topical treatment of cutaneous ptosis: Endolift® treatment with 1470-nm wavelength Eufoton® LASEmaR® 1500. J Appl Cosmetol 2023;41:45-8. DOI: https://doi.org/10.56609/jac.v41i1.69
22. Dias L, Almeida D, dos Santos Borges F, et al. The 1470 nm diode laser effectiveness in facial fat reduction with the endolifting technique: pilot study. Int J Med Sci Clin Invent 2023;10:6788-95. DOI: https://doi.org/10.18535/ijmsci/v10i6.02
23. Leclère FM, Alcolea JM, Vogt P, et al. Laser-assisted lipolysis for arm contouring in Teimourian grades I and II: a prospective study of 45 patients. Lasers Med Sci 2015:30;1053-9. DOI: https://doi.org/10.1007/s10103-014-1705-2
24. Vidal GP, Parizotto NA, Borges FDS, et al. Scientific evidence on the impact of endolaser in aesthetics: scoping review. Lasers Med Sci 2025;40:155. DOI: https://doi.org/10.1007/s10103-024-04248-z
25. Li K, Nicoli F, Xi WJ, et al. The 1470 nm diode laser with an intralesional fiber device: a proposed solution for the treatment of inflamed and infected keloids. Burns Trauma 2019;7:5. DOI: https://doi.org/10.1186/s41038-019-0143-6
26. Kubik P, Bighetti S, Bettolini L, et al. Effectiveness and safety of the use of 1470 nm laser therapy in patients suffering from acne scarring of the facial skin. Clin Cosmet Investig Dermatol 2025;18:543-51. DOI: https://doi.org/10.2147/CCID.S510208
27. Liu H, Dang Y, Wang Z, et al. Laser induced collagen remodeling: a comparative study in vivo on mouse model. Lasers Surg Med 2008;40:13-9. DOI: https://doi.org/10.1002/lsm.20587
28. Beigvand HH, Razzaghi M, Rostami-Nejad M, et al. Assessment of laser effects on skin rejuvenation. J Lasers Med Sci 2020;11:212-9. DOI: https://doi.org/10.34172/jlms.2020.35
29. Di Bernardo, Barry E, Reyes, J. Preliminary report: evaluation of skin tightening after laser-assisted liposuction. Aesthet Surg J 2009;29:400-7. DOI: https://doi.org/10.1016/j.asj.2009.08.006
30. Godoi S, Sant’Ana E, Renno ACM, et al. Evaluation of the photothermal effects of the subdermal high-power laser in the skin of an experimental rat model. Lasers Med Sci 2024:39:230. DOI: https://doi.org/10.1007/s10103-024-04182-0
31. Levi JR, Veerappan A, Chen B, et al. Histologic evaluation of laser lipolysis comparing continuous wave vs pulsed lasers in an in vivo pig model. Arch Facial Plast Surg 2011;13:41-50. DOI: https://doi.org/10.1001/archfaci.2010.103
32. Saldanha O, Ordenes AI, Goyeneche C, et al. Lipoabdominoplasty with anatomic definition: an evolution on Saldanha’s technique. Clin Plast Surg 2020;47:335-49. DOI: https://doi.org/10.1016/j.cps.2020.03.004
33. Matarasso A, Matarasso DM, Matarasso EJ. Abdominoplasty: classic principles and technique. Clin Plast Surg 2014;41:655-72. DOI: https://doi.org/10.1016/j.cps.2014.07.005
34. Goyal NN. Tumescent anaesthesia for liposuction surgery—A review. Dermatol Rev 2021;2:180-7. DOI: https://doi.org/10.1002/der2.80
35. Chen Y, Huang J, Wang K, et al. Experimental research on mechanism of collagen remodeling and excellent properties of skin incisions under dual beam laser welding. Opt Laser Technol 2024;169:110168. DOI: https://doi.org/10.1016/j.optlastec.2023.110168
36. Baidoo N, Crawley E, Knowles CH, et al. Total collagen content and distribution is increased in human colon during advancing age. PloS one 2022:17:e0269689. DOI: https://doi.org/10.1371/journal.pone.0269689
37. Pirri C, Fede C, Petrelli L, et al. Elastic Fibres in the subcutaneous tissue: Is there a difference between superficial and muscular fascia? A cadaver study. Skin Res Technol 2022;28:21-7. DOI: https://doi.org/10.1111/srt.13084
38. Couto SR, Luan X, Rossmann JA, et al. An in vivo comparison of wound healing characteristics of two commercial acellular dermal matrices. Clin Exp Dent Res 2021;7:679-91. DOI: https://doi.org/10.1002/cre2.412
39. Lattouf R, Younes R, Lutomski D, et al. Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues. J Histochem Cytochem 2014;62:751-8. DOI: https://doi.org/10.1369/0022155414545787
40. Coelho PGB, Souza MVD, Conceição LG, et al. Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy. An Bras Dermatol 2018;93:415-8. DOI: https://doi.org/10.1590/abd1806-4841.20187544
41. Lovell CR, Smolenski KA, Duance VC, et al. Type I and III collagen content and fibre distribution in normal human skin during ageing. Br J Dermatol 1987;117:419-28. DOI: https://doi.org/10.1111/j.1365-2133.1987.tb04921.x
42. Min KH, Kim JH, Park HJ, et al. The skin-tightening effects of 1,444-nm Nd: YAG laser on human skin: an in vivo study. Aesthetic Plast Surg 2014;38:585-91. DOI: https://doi.org/10.1007/s00266-014-0316-4
43. Yoon J, Park JH, Choi JW, et al. Optimal Fluence and Duration of Low-Level Laser Therapy for Efficient Wound Healing in Mice. Ann Dermatol 2021;33:318-23. DOI: https://doi.org/10.5021/ad.2021.33.4.318
44. Avci P, Gupta A, Sadasivam M, et al. Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 2013;32:41-52.
45. Gurtner G, Werner S, Barrandon Y, et al. Wound repair and regeneration. Nature 2008;453:314-21. DOI: https://doi.org/10.1038/nature07039
46. Tierney EP, Kouba DJ, Hanke CW. Review of fractional photothermolysis: treatment indications and efficacy. Dermatol Surg 2009;35:1445-61. DOI: https://doi.org/10.1111/j.1524-4725.2009.01258.x
Ethics Approval
The study followed the principles of the Declaration of Helsinki and was approved by the Ethics Committee of the University of Turin (no. 0271617 - 08/06/2022 - [UOR: SI000045 - Classif. III/11]). All patients provided written informed consent to participate and to share their anonymous data, photography, and skin biopsy procedures (excision explants) in this study.CRediT authorship contribution
Daniele Bollero: patient inclusion, surgical and laser treatment, skin samples collection; Daniele Bollero, Roberto Dell’Avanzato, Bruno Bovani, Riccardo Forte: methodology, clinical data conceptualization; Rebecca Senetta, Giulia Orlando, Irene Cambieri, Stefania Minzon, Sara Scutera: sample processing, histological analysis validation, data curation; Tiziana Musso, Rebecca Senetta, Sara Scutera: validation, formal analysis, investigation, writing – review & editing, supervision. All the authors have read and approved the final version of the manuscript and agreed to be accountable for all aspects of the work.
Supporting Agencies
This study was funded by the University of Turin (Local Funds TM).Data Availability Statement
Data are available upon reasonable request from the corresponding author.
How to Cite
In vivo pilot study of the effects of a subdermal 1470 nm diode laser on human skin. (2025). Laser Therapy, 32(1). https://doi.org/10.4081/ltj.2025.422
Copyright (c) 2025 the Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.