Original Research Articles
30 June 2025
Vol. 32 No. 1 (2025)
https://doi.org/10.4081/ltj.2025.422

In vivo pilot study of the effects of a subdermal 1470 nm diode laser on human skin

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Diode laser therapy, Endolift®, skin tightening, lipolysis
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Authors

Daniele Bollero
Center for Orthopedic Trauma Hospital, Turin, Italy.
Giulia Orlando
Pathology Unit, Department of Oncology, University of Turin, Italy.
Roberto Dell'Avanzato
La Madonnina Clinic, Milan, Italy.
Irene Cambieri
Skin Bank, Department of General and Specialized Surgery, AOU Città della Salute e della Scienza, Turin, Italy.
Bruno Bovani
Studio Medico Esculapio, Perugia, Italy.
Stefania Minzon
Skin Bank, Department of General and Specialized Surgery, AOU Città della Salute e della Scienza, Turin, Italy.
Riccardo Forte
RF Clinics, Como, Italy.
Rebecca Senetta
Pathology Unit, Department of Oncology, University of Turin, Italy.
Sara Scutera
sara.scutera@unito.it
https://orcid.org/0000-0002-7431-0875
Department of Public Health and Pediatric Sciences, University of Turin, Italy.
Tiziana Musso
Department of Public Health and Pediatric Sciences, University of Turin, Italy.

This in vivo pilot study investigates the histological regenerative effects of a subdermal 1470 nm laser (EndoliftX®, Eufoton® , Trieste, Italy) on human abdominal skin, aiming to assess its role in dermal remodeling. Laser energy was delivered at 5, 20, and 40 J/cm2 to defined areas of abdominal skin in patients undergoing abdominoplasty. Biopsies were obtained at 3 and 6 months post-treatment and assessed for changes in dermal thickness, collagen, and elastin content compared to the control untreated region. Histological staining and image analysis revealed that the 5 and 20 J/cm2 treatments significantly increased dermal thickness and collagen deposition, particularly with the 20 J/cm2 dose, which induced dense, organized collagen bundles indicative of neocollagenesis. Sirius red staining showed increased type III collagen, and Verhoeff-Van Gieson (VVG) staining indicated enhanced and more aligned elastin fibers at moderate energy levels. Conversely, the 40 J/cm2 treatment dose showed signs of collagen fragmentation and reduced elastin coherence, suggesting potential thermal damage. These findings confirm the efficacy of EndoliftX in promoting skin tightening and remodeling at optimal energy settings while highlighting a non-linear dose-response relationship. The results support further development of personalized protocols for minimally invasive procedures in regenerative medicine and aesthetic dermatology.

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Supporting Agencies

This study was funded by the University of Turin (Local Funds TM).

How to Cite



In vivo pilot study of the effects of a subdermal 1470 nm diode laser on human skin. (2025). Laser Therapy, 32(1). https://doi.org/10.4081/ltj.2025.422
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